National Journal of Biological Sciences (NJBS)

Authors:   Aneeqa Shafqat ^[1], Sumbal Nazir ^[2], Ishrat Perveen ^[3], Muhammad Usama Rehman ^[4],
DOI: https://doi.org/10.37605/v6i2/1

Abstract

Objectives: Bacterial colonies proliferate in wounds and cuts of burn infections. These bacterial strains are the main cause of burn infections because they enter the body and multiply, leading to infection. The present study was to isolate the bacterial strains from the burn infected patients and also find the antibiotic susceptibility of the isolated bacterial strains.

Methods: Eight bacterial strains i.e., Staphylococcus aureus, Klebsiella oxytoca, P. aeruginosa, Coagulase negative Staphylococcus species, Proteus mirabilis,MRSA, and other Pseudomonas species were detected from burn wounds infections. Fifteen burn patients of both sexes had their burn wounds sampled. The average age of the four female and eleven male patients hospitalized in burn unit of Jinnah hospital Lahore was between 17 and 58 years old.

Results: The best dominant bacterial species was Staphylococcus aureus (90.90%) followed by Coagulase-negative Staphylococcus species (36.36%), Proteus mirabilis (36.36%), Pseudomonas aeruginosa (54.54%), Klebsiella oxytoca (63.63%), Methicillin resistant Staphylococcus aureus (MRSA) (18.18%), Pseudomonas species (54.54%). Antimicrobial susceptibility testing was carried out to the burn infections bacterial isolates. Gentamicin was found to be the most effective drug against most of the Gram positive and Gram negative isolates Tetracycline least effective. Amikacin and Ciprofloxacin showed highest resistance, and Tetracycline and Chloramphenicol showed lowest resistance against burn wound infections. Staphylococcus aureus showed Pseudomonas species or Methicillin-resistant Staphylococcus aureus (81.81%) had the highest percent for extensive drug resistance and multi drug resistance (90.90%) among the bacteria species. No Pan drug resistant (PDR) were observed in isolated bacterial strains of burn